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Paradoxical effects of kisspeptin: it enhances oocyte in vitro maturation but has an adverse impact on hatched blastocysts during in vitro culture
Faculty
Veterinary Medicine
Year:
2012
Type of Publication:
Article
Pages:
656-668
Authors:
Saadeldin, Islam M, Jang, Goo, Oh, Hyun Ju, Lee, Byeong Chun, Koo, Ok Jae, Kang, Jung Taek, Kwon, Dae Kee, Park, Sol Ji, Kim, Su Jin, Moon, Joon Ho
DOI:
10.1071/RD11118
Journal:
REPRODUCTION FERTILITY AND DEVELOPMENT CSIRO PUBLISHING
Volume:
24
Research Area:
Developmental Biology; Reproductive Biology; Zoology
ISSN
ISI:000304603300003
Keywords :
embryo development, KISS1R, oocyte maturation, p234
Abstract:
Kisspeptin (Kp) is best known as a multifunctional peptide with roles in reproduction, the cardiovascular system and cancer. In the present study the expression of kisspeptin hierarchy elements (KISS1, GNRH1 and LHB) and their receptors (KISS1R, GNRHR and LHCGR, respectively) in porcine ovary and in cumulus-oocyte complexes (COCs) were investigated, as were its effects on the in vitro maturation (IVM) of oocytes and their subsequent ability to sustain preimplantation embryo competence after parthenogenetic electrical activation. Kp system elements were expressed and affected IVM of oocytes when maturation medium was supplemented with 10(-6) M Kp. Oocyte maturation, maternal gene expression (MOS, GDF9 and BMP15), blastocyst formation rate, blastocyst hatching and blastocyst total cell count were all significantly increased when oocytes were matured in medium containing Kp compared with the control group (without Kp). A Kp antagonist (p234) at 4 x 10(-6) M interfered with this hierarchy but did not influence the threshold effect of gonadotrophins on oocyte maturation. FSH was critical and permissive to Kp action on COCs by increasing the relative expression of KISS1R. In contrast, Kp significantly increased apoptosis, the expression of pro-apoptotic gene, BAK1, and suppressed trophoblast outgrowths from hatched blastocysts cultured on feeder cells. The present study provides the first functional evidence of the Kp hierarchy in porcine COCs and its role in enhancing oocyte maturation and subsequent developmental competence in an autocrine-paracrine manner. However, Kp supplementation may have a harmful impact on cultured hatched blastocysts reflecting systemic or local regulation during the critical early period of embryonic development.
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