Myeloperoxidase As A Marker Of Inflammation In Asthmatic Patients

the association of bronchial asthma with the release of inflammatory mediators, through a mechanism that involves the immune system is a subject of every day vivid study. The inflammatory mediators, particularly those derived from neutrophil and its role in airway inflammation in bronchial asthma are still under discussion.This study was undertaken to assess the serum and local (BAL fluid) MPO level as an inflammatory mediator involved in asthma pathogenesis and its relationship to disease severity.This study included thirty six asthmatic patients (extrinsic and intrinsic) and ten apparently healthy control subjects.All patients and control subjects were subjected to the following:1) Computerized pulmonary function (sensor medics 2450) testing in the form of flow volume curve data before and 20 minutes after inhaled salbutamol 400 ug via MDI and a spacer.2) Urine and stool analysis.3) Skin prick test.4) Peak expiratory flow rate using a mini Wright PEF meter.5) Complete blood picture for determination of total and differential leucocytic counts.9) Estimation of serum MPO level using Elisa kit and estimation of serum urea.10) Fibroptic bronchoscopy (Olympus model BF type) and broncholaveolar lavage fluid was examined for:1- MPO level using Elisa kit (Oxis International Inc., Protland, OR).2- Total and differential leucocytic count.3- Estimation of urea level.