DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction

Faculty Science Year: 2014
Type of Publication: ZU Hosted Pages: 1-14
Journal: Front. Microbiol. (Frontiers of Microbiology) PubMed Volume: 5
Keywords : , polymerase hybrids derived from , family-B enzymes    
The polymerase chain reaction (PCR) is widely applied across the biosciences, with archaeal Family-B DNA polymerases being preferred, due to their high thermostability and fidelity. The enzyme from Pyrococcus furiosus (Pfu-Pol) is more freq