Monitoring The Effect Of Therapy On The Presence Of BCR-ABL Fusion Gene In Patients With Chroing Myeloid Leukamia

Chronic myelogenous leukemia is a hematopietic stem cell disease which is unique among Chronic myeloproliferative disorders because of its association with a t (9; 22) q34 q 11.2) or a variant of this anomaly. This reciprocal transtocation (ph chromosome) creates a fusion of portions of the ABL oncogene from 9q34 and the BCR gene atq11.2. karyotype analysis, for various reasons, has remained the ”gold standard” method for detection of ph chromosome but is relatively time consummg.Recent advances in genetic methodology have led to newchallenges, real-time RT-Pf’R is on of the most developing techniques that can be used for detection of ph chromosome or its variants. However, when introducing a new diagnostic tool, it is necessary to carefully evaluate it in comparison with the long proven standard technique.We were selected 20 patients suffering from CML from outpatients and in-patients of Zagazig University; 12 were males (ranged from 22-50 years) with a mean of 41.4 y. and 8 were females (ranged from 28- 51 years) with a mean of37.75 years.All the symptoms were [abdominal discomfort (frequency 92.5 %), anorexia (freq.65%), fatigue (freq. 65%), heat intolerance (rreq.60 %), easy and bony pain (freq.40%), urticaria (freq.46%), weight loss (freq.300/c,) and night sweat (ticq.20 %).