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Spectrofluorimetric and Spectrophotometric Analysis of Two Analgesic Drugs in Pharmaceutical Formulations and Biological Fluids
Faculty
Science
Year:
2013
Type of Publication:
Article
Pages:
1322-1329
Authors:
El-Didamony, Akram M, Ali, Ismail I
DOI:
10.1111/1556-4029.12170
Journal:
JOURNAL OF FORENSIC SCIENCES WILEY-BLACKWELL
Volume:
58
Research Area:
Legal Medicine
ISSN
ISI:000323894200031
Keywords :
forensic science, spectrofluorimetry, spectrophotometry, analgesic drugs, Ce(IV), pharmaceutical formulations, biological samples
Abstract:
Simple, reliable, sensitive, and accurate spectrofluorimetric and spectrophotometric methods were proposed for the determination of two selected analgesic drugs, namely tramadol and morphine, in pharmaceuticals and biological fluids. The proposed methods were based on the oxidation of the studied drugs by Cerium (Ce)IV in an acidic medium. The spectrofluorimetric method is based on measuring the relative fluorescence intensity of Ce(III) arising from Ce(IV) at 350nm with an excitation wavelength at 250nm. The spectrophotometric method involves on addition of a known excess of Ce(IV) to the studied drugs in an acid medium, followed by the determination of residual Ce(IV) by reacting with a fixed amount of methyl orange and measuring the absorbance at 510nm. Different variables affecting the reaction conditions, such as the concentrations of Ce(IV), type and concentration of the acid medium, reaction time, temperature, and the diluting solvents, were carefully studied and optimized.
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