One pot Synthesis, DNA binding and fragmentation in vitro of new fused uracil derivatives for anticancer properties

A new series of pyrimidopyrimidines are synthesized starting from 6-amino-1-benzyl{[}or1-(2-chlorobenzyl)]uracil via the formation of nonisolable acyclic Michael type adduct followed by nucleophilic addition and finally aromatization. The same starting material can be used for the preparation of some new pyrimidodipyrimidines by refluxing with different aldehydes in slightly basic medium. Finally different lumazines are synthesized via S-N(2) type reaction of diaminouracil hydrochloride with phenacyl bromides followed by intramolecular cyclodehydration then aromatization. The newly synthesized compounds showed binding, chelation and fragmentation of the nucleic acid DNA.