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The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding
Faculty
Veterinary Medicine
Year:
2012
Type of Publication:
Article
Pages:
Authors:
Azab, Walid, Osterrieder, Nikolaus, Zajic, Lara
DOI:
10.1186/1297-9716-43-61
Journal:
VETERINARY RESEARCH BIOMED CENTRAL LTD
Volume:
43
Research Area:
Veterinary Sciences
ISSN
ISI:000312634400001
Keywords :
, role , glycoprotein , , equine herpesviruses , , , (EHV-1 , EHV-4)
Abstract:
Equine herpesvirus type 1 and 4 (EHV-1 and EHV-4) glycoprotein H (gH) has been hypothesized to play a role in direct fusion of the virus envelope with cellular membranes. To investigate gH's role in infection, an EHV-1 mutant lacking gH was created and the gH genes were exchanged between EHV-1 and EHV-4 to determine if gH affects cellular entry and/or host range. In addition, a serine-aspartic acid-isoleucine (SDI) integrin-binding motif present in EHV-1 gH was mutated as it was presumed important in cell entry mediated by binding to alpha 4 beta 1 or alpha 4 beta 7 integrins. We here document that gH is essential for EHV-1 replication, plays a role in cell-to-cell spread and significantly affects plaque size and growth kinetics. Moreover, we could show that alpha 4 beta 1 and alpha 4 beta 7 integrins are not essential for viral entry of EHV-1 and EHV-4, and that viral entry is not affected in equine cells when the integrins are inaccessible.
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