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COLORIMETRIC METHOD FOR THE QUANTITATIVE-DETERMINATION OF THE ANTIBILHARZIAL DRUG PRAZIQUANTEL AND ITS APPLICATION TO PHARMACEUTICAL PREPARATIONS
Faculty
Pharmacy
Year:
1992
Type of Publication:
Article
Pages:
87-92
Authors:
SALEH, H, SCHNEKENBURGER, J
DOI:
10.1039/an9921700087
Journal:
ANALYST ROYAL SOC CHEMISTRY
Volume:
117
Research Area:
Chemistry
ISSN
ISI:A1992GX13400019
Keywords :
4-CHLORO-7-NITRO-2, 1, 3-BENZOXADIAZOLE, COLORIMETRY, PRAZIQUANTEL DETERMINATION
Abstract:
A method for the colorimetric assay of praziquantel has been developed. For the colorimetric assay, it was necessary to hydrolyse praziquantel with 3 mol dm-3 NaOH, 6 mol dm-3 HCl and 85\% phosphoric acid separately. 4-Chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) reacts with the basic hydrolysis product in methanolic aqueous phosphate buffer (pH 7.4), resulting in the formation of an orange product with a characteristic absorption maximum at 478 nm. The red-orange product of the interaction between the hydrochloric acid hydrolysis product and NBD-Cl showed an absorption maximum at 486 nm. The colours obtained were stable for 24 h. The colour system obeyed Beer's law in the concentration range 2-15 and 2-18 mu-g ml-1 for the basic hydrolysis product and the acid hydrolysis product, respectively. The results obtained showed good recoveries with relative standard deviations of 0.378 and 0.47\% for the basic and the acid hydrolysis product, respectively. The determination limit was found to be 0.124 and 0.150-mu-g ml-1 for the praziquantel basic hydrolysis product and the acid hydrolysis product, respectively. The coloured reaction products obtained with the proposed method were synthesized. The structures of these products were studied and the compounds identified.
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