Genetic Polymorphism of Quantitative Treat Loci in Egyption Buffalo (Bublus bublis I)

River buffalo is a species of great economically importance, it is amajor source of milk and meat in Egypt. The improvement of Egyptianbuffalo productivity has been dependent on DNA and genetic markersthat are associated with various productivity traits of economicimportance. The meat and milk quality and quantity characteristics werereported to be associated with the genetic polymorphisms of some genesincluding; leptin (LEP) , u-calpain (CAPNi), pituitary-specifictranscription factor (PIT-i), prolactin (PRL) and kappa-casein (K-CN).PCR-RFLP technique was used in this study to detect the geneticpolymorphisms of these five genes in Egyptian river buffalo. PCRamplified buffalo DNA using the specific primers for the five testedgenes. The PCR products of these genes were digested with specificrestriction enzymes; leptin (Sau3AI), u-calpain (FakI), pituitary-specifictranscription factor (Hinfl), prolactin (RsaI) and kappa-casein (HindlII).The results showed that all tested Egyptian buffaloes possesshomozygous genotypes for the five studied genes; AA for leptin gene, CCfor u-calpain gene, BB for pituitary-specific transcription factor gene, GGfor prolactin gene and BB for kappa-casein gene.