RADIOIODINATION OF SOME ORGANIC COMPOUNDS USING Na125I VIA ELECTROPHILIC OR NUCLEOPHILIC SUBSTITUTION REACTION

The objective of this thesis can be divided into three parts.the first part deal with the invistigation of optimum conditions for labeling of Evan blue via electrophilic substitution reaction. The second part involves the nucleophilic radioiodination of o1- Electrophilic radiiodination of Evan blue was carried out by using several oxidizing agents such as chloramine-T, iodegen and N- bromosuccinamide. The factors affecting the percent of the radiochemical yield such as substrate concentration, oxidizing aThe stability of the purified labeled compound (125IEB) was carried out to determine the suitable time of injection at which no side products can be occurred.2- (a) A nucleophilic radioiodination of o-iodohippuric acid (o-IHA) has been investigated. Dimethylformamide (DMF) has been used as a solvent. Hippuran contains CONHCH2COOH moiety, which promotes tubular excretion of organic anions. Different parameters (b) Separation and purification of the product (o-125IHA) was achieved by high performance liquid chromatography (HPLC) under special condition.3- Using the dry state on m-IHA, a yield of about 93.6%, at 165 °C was obtained by using 2 mg of tetrabutyl ammonium bromide (TBAB). The activation energy of the exchange reaction between m-IHA and Na125I, without catalyst and in presence of TABAB in dry