| Abstract: |
SUMMARYSurgical site infection (SSI) as a type of nosocomial infection (NI) remains an important cause of morbidity and mortality among hospitalized patients.So as to the aim of this study was to determine the rate of nosocomial infection (NI) and identify the associated risk factors, studying the susceptibility patterns of different clinical bacterial strains in order to evaluation of the infection control meThis study included 254 bacterial isolates recovered from 303 clinical samples collected from 173 inpatients in the Department of General Surgery in Zagazig University Hospitals suffering from different bacterial infections.Bacterial cultures were isolated and grown on different selective media to investigate their morphological and cultural characteristics. Standard bacteriological identification was done for all isolates by microscopical, cultural and biochemical methods. The results of this study can be summarized as follows:1- A total of 303 surgical specimens were collected from 173 inpatients in the Department of General Surgery in Zagazig University Hospitals during the period from June 2001 to July 2002.2- A total of 254 bacterial isolates were isolated from the surgical specimens as nosocomial infection (NI) causative organisms.3- Age, gender, surgical interference and diagnosis of each case are considered as risk factors associated with NI.4- A percentage of (80.9%) of total clinical specimens were contaminated with different bacterial infections causing NI.5- Identification and confirmation of the studied groups of bacterial isolates using biochemical tests revealed that the most dominant bacteria isolated causing NI were: (37%) Staph. aureus, (25.2%) P. aeruginosa, (11.4%) K. pneumoniae, (10.2%) E. coli, (6- Diabetic foot cases revealed high contamination with Staph. aureus (20.2 %) followed by P. aeruginosa (12.6 %) and then P. vulgaris (5.2 %).7- Abdominal operation cases were highly contaminated with E. coli and K. pneumoniae (5.8 %).8- The mean of age of patients of abscesses and ulcers cases who are susceptible to NI ranged between 24 – 48 years; in case of diabetic foot cases ranged between 36.33 to 41.75 years; in potentially contaminated operations ranged between 33 – 49.50 years9- Males are more susceptible to have infection by different causative organisms where 139 out of 254 infectious bacteria corresponding to 55 % of total isolates were isolated from male patients than females’ only 115 out of 254 infectious bacteria corres10- Concerning surgical interference, the post operative cases represented 56% of total infected cases while 44% of infection occurred in preoperative cases.11- Screening the different bacterial strains for their antibiotic susceptibility revealed that Imipenem proved to have a broad spectrum and high activity against all tested strains.12- Determination of minimum inhibitory concentrations (MIC) of Imipenem using E-test against most resistant bacterial strains; MRSA (6), E. coli (7), P. vulgaris (4), K. pneumoniae (3) and P. aeruginosa (34) were 1, 0.125, 0.19, 0.25 and 0.75 µg / ml res13- Plasmid profile of 7 chosen multidrug resistant MRSA strains (M1,M2,M3,M4,M5,M6 and M7) revealed that the presence of plasmids in the seven strains by gel electrophoresis using λ Hind III DNA marker were of sizes ranging between 25 – 45.934 Kbp.
|
|
|