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IMMUNOPATHOLOGICAL AND HISTOCHEMICAL STUDIES ON HUMAN PREGNANT MOTHERS AND THEIR NEWLY BORN BABIES IN ACUTE TOXOPLASMA INFECTION
Faculty
Science
Year:
2004
Type of Publication:
Theses
Pages:
Authors:
NIVIN SALAH EL-DIN SALEM MOHAMMED
BibID
9979447
Keywords :
HUMAN PREGNANT MOTHERS AND THEIR NEWLY BORN BABIES
Abstract:
An interest has been focused on evaluating certain diagnostic methods to differentiate acute from harmless chronic toxoplasmosis. The present work was carried on 110 cases; 80 women with positive ELISA IgM, 20 children belonged to the same women and 5 hea1- ELISA:Application of toxoplasma minor serologic profile reclassified the 80 women into acute (47) cases and chronic (33) cases. 17 babies were congenitally infected and three were toxoplasma-free.2- PCR:It was applied to qualify the use of ELISA IgA as a high specific, sensitive and accurate marker to diagnose congenital infection.3- Macroscopic examination of placenta:There were no statistical differences between serpositive and control placentas or between acute and chronic placentas.4- Microscopic examination of placenta:High index of suspicious was obtained by means of light microscopy alone. Villous immaturity may reflect toxoplasmosis.5- PAS:There was no T.gondii organism could be detected by PAS stain. Another special stain was recommended.6- PAP against B-hCG:There was no change in the secretion of B-hCG accompanying toxoplasmosis.7- Image analysis:All of the seropositive and control placentas were diploid and the SPF range between low and relatively high.In conclusion, the application of screening programs is recommended to avoid acute toxoplasmosis among the pregnant women. The application of toxoplasma minor serologic profile will provide very good results to differentiate between acute and chronic toxo
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