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CHARACTERIZATION OF HAEMOLYSER-RESISTANT CELLS INCREASED IN THE BLOOD OF ERYTHROPOIETIN-TREATED MICE
Faculty
Veterinary Medicine
Year:
1994
Type of Publication:
Article
Pages:
361-365
Authors:
SAID, AA, YAMAGUCHI, T, UCHIDA, E, HAYAKAWA, T
DOI:
10.1111/j.1365-2141.1994.tb04740.x
Journal:
BRITISH JOURNAL OF HAEMATOLOGY BLACKWELL SCIENCE LTD
Volume:
86
Research Area:
Hematology
ISSN
ISI:A1994MW06200020
Keywords :
ERYTHROPOIETIN, EPO BIOASSAY, EPO-INDUCED CELLS, HAEMOLYSER-RESISTANT CELLS, IMMATURE RETICULOCYTES
Abstract:
Recently, we reported a new in vivo assay method for erythropoietin (Epo) by means of counts of the number of haemolyser-resistant cells (HRCs) increased in Epo-injected mice. Here, we attempted to characterize the HRCs. Flow-cytometric studies revealed that HRCs obtained from Epo-injected mice expressed the transferrin receptor on their surface membranes. Furthermore, a fluorophotometric study suggested that the number of transferrin receptor-positive cells increased in a dose-dependent manner in response to treatment with Epo. On the other hand, flowcytometric and fluorophotometric studies of glycophorin A on HRCs using a rabbit antiglycophorin A antibody also showed a high expression of glycophorin A on them as compared with on HRCs from vehicle-treated animals (control). The results indicated that HRCs could be defined by their expression of both transferrin receptors and glycophorin A. We concluded that HRCs might be immature reticulocytes.
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